The Amersham ECL Detection Reagents, 4000 cm² (RPN2105) are enhanced chemiluminescent detection reagents for HRP-based blot detection workflows. This pack is sufficient for 4000 cm² of membrane and contains two detection reagents that are mixed immediately before use.
Key Features
- Enhanced luminol-based chemiluminescence, supporting HRP-based detection for routine blotting workflows
- 4000 cm² membrane coverage, supplied as the RPN2105 pack size for larger membrane processing needs
- Two-reagent detection system, containing 250 mL detection reagent 1 and 250 mL detection reagent 2
- Fresh working solution preparation, with equal volumes of detection solution 1 and detection solution 2 mixed immediately before use
- Efficient membrane coverage guidance, with a final working volume of 0.125 mL/cm² membrane specified in the manufacturer protocol
- HRP substrate chemistry, using hydrogen peroxide generation and luminol oxidation to produce detectable light output
- Fast detection workflow, with a 1 minute membrane incubation step specified in the protocol and rapid light output for imaging
- Refrigerated storage, specified at 2 to 8 °C before use
Benefits
- Supports routine confirmatory Western blotting and other HRP-labelled blot detection workflows.
- Large membrane coverage helps laboratories process multiple blots from a single reagent pack.
- Two-solution format allows the working detection reagent to be prepared fresh when required.
- Chemiluminescent detection provides a non-radioactive approach for sensitive blot visualisation.
- Clear reagent volume guidance helps users plan membrane coverage and reduce avoidable reagent waste.
Why Choose the Amersham ECL Detection Reagents, 4000 cm² (RPN2105)?
The Amersham ECL Detection Reagents, 4000 cm² (RPN2105) provide a verified ECL chemiluminescent reagent format for HRP-based membrane detection. John Morris Group supplies Amersham protein analysis and blotting reagents across Australia with support for product selection and ordering.
