HiScreen Ni FF Column, 4.7 mL (28978244) is a prepacked IMAC column for method optimisation and parameter screening in histidine-tagged protein purification. The column is packed with Ni Sepharose 6 Fast Flow and provides a 4.7 mL bed volume in a 0.77 × 10 cm HiScreen format.
Key Features
- Prepacked IMAC column format, designed for convenient purification screening of histidine-tagged proteins
- Ni Sepharose 6 Fast Flow resin, using 90 µm beads of highly cross-linked 6% agarose with an immobilised chelating ligand
- 4.7 mL column volume, supplied as a single prepacked column for compact method development workflows
- 10 cm bed height, with column dimensions of 0.77 × 10 cm
- High binding capacity for tagged proteins, with approximately 40 mg (his)6-tagged protein/mL resin
- Defined metal ion capacity, with approximately 15 µmol Ni2+/mL resin
- Broad operating flow range, with recommended operating flow velocity from 30 to 300 cm/h
- Controlled storage conditions, stored at 4°C to 30°C in 20% ethanol
Benefits
- Supports efficient screening of binding and elution conditions for histidine-tagged recombinant proteins.
- Prepacked format helps improve convenience and reproducibility compared with manually packed columns.
- Small column volume helps reduce sample and buffer consumption during process development.
- Compatible with peristaltic pumps and chromatography systems such as ÄKTA systems.
- Can be connected in series with another HiScreen column to achieve a 20 cm bed height when required.
Why Choose the HiScreen Ni FF Column, 4.7 mL (28978244)?
HiScreen Ni FF Column, 4.7 mL (28978244) is a practical choice for laboratories developing IMAC purification methods using Ni Sepharose 6 Fast Flow. John Morris Group can assist with Cytiva chromatography consumables and related protein purification requirements for Australian laboratories.
