HiScreen Blue FF Prepacked Column, 4.7 mL (28978243) is a ready-to-use HiScreen column prepacked with Blue Sepharose 6 Fast Flow resin for affinity chromatography method optimisation and parameter screening. The 4.7 mL column format is designed for small-scale purification work, including albumin depletion and purification of proteins such as interferons, lipoproteins, coagulation factors, kinases, dehydrogenases, and enzymes requiring adenyl-containing cofactors.
Key Features
- Prepacked Blue Sepharose 6 Fast Flow format, ready for convenient method development and screening
- 4.7 mL HiScreen column volume, helping reduce sample and buffer consumption during process development
- 10 cm bed height, designed to support method optimisation and parameter screening
- Cibacron Blue 3G-A ligand chemistry, covalently attached to the Sepharose 6 Fast Flow matrix by the triazine coupling method
- ≥ 18 mg HSA/mL resin dynamic binding capacity, supporting albumin-focused affinity chromatography workflows
- 90 µm median particle size, based on cross-linked 6% spherical agarose resin
- Recommended operating flow velocity of 30 to 300 cm/h, with maximum operating flow velocity of 410 cm/h
- Series connection option, allowing two HiScreen columns to be connected to achieve a 20 cm bed height
Benefits
- Helps laboratories screen binding and elution conditions before moving to larger process chromatography formats.
- Supports fast, lower-volume method development where reducing sample and buffer use is important.
- Provides a practical format for albumin depletion and purification of dye-binding proteins.
- Supports reproducible scale-up using the same resin and linear flow velocity principles.
Why Choose the HiScreen Blue FF Prepacked Column, 4.7 mL (28978243)?
HiScreen Blue FF Prepacked Column, 4.7 mL (28978243) provides a compact Blue Sepharose 6 Fast Flow format for affinity chromatography screening and small-scale protein purification. John Morris Group supplies this Cytiva consumable for Australian laboratories developing reliable biomolecule purification workflows.
