The HiScreen Octyl FF Column, 4.7 mL (28926986) is a prepacked hydrophobic interaction chromatography column containing Octyl Sepharose 4 Fast Flow medium. It is supplied as 1 x 4.7 mL for HIC method optimisation, parameter screening, capture and intermediate purification workflows.
Key Features
- Prepacked HIC format, supplied ready to use for convenient process development optimisation
- 4.7 mL bed volume, helping reduce sample and buffer consumption during screening
- 10 cm bed height, designed to support method optimisation and parameter screening
- Octyl ligand chemistry, using Octyl Sepharose 4 Fast Flow for hydrophobic interaction chromatography
- 4% cross-linked agarose matrix, supplied as spherical particles for the packed resin bed
- 5 µmol/mL ligand density, with a median particle size of 90 µm
- 1.2 mL/min recommended flow rate, with a listed maximum flow rate of 1.9 mL/min
- Operational pH stability from 3 to 13, with storage specified at 4°C to 30°C in 20% ethanol
Benefits
- Supports efficient screening of hydrophobic interaction chromatography conditions before scale-up.
- Helps conserve valuable samples and buffers by using a compact 4.7 mL column bed.
- Allows two columns to be connected in series when a 20 cm bed height is required.
- Provides a convenient prepacked format that reduces manual packing time and setup variability.
- Offers a complementary octyl selectivity compared with phenyl and butyl HIC ligand chemistries.
Why Choose the HiScreen Octyl FF Column, 4.7 mL (28926986)?
Choose the HiScreen Octyl FF Column, 4.7 mL (28926986) when you need a compact, prepacked HIC column for screening and optimisation using Octyl Sepharose 4 Fast Flow. John Morris Group can support Australian laboratories with supply of Cytiva chromatography consumables and related workflow requirements.
