The HiTrap Capto Phenyl ImpRes Column, 5 x 1 mL (17548411), is a pack of prepacked chromatography columns for screening and small-scale protein purification using hydrophobic interaction chromatography. Each 1 mL column is prepacked with BioProcess Capto Phenyl ImpRes resin, supporting selectivity, binding and elution condition screening.
Key Features
- Pack of five 1 mL columns, supplied as 5 x 1 mL for small-scale purification and method screening
- Hydrophobic interaction chromatography format, designed for protein purification using HIC workflows
- Prepacked Capto Phenyl ImpRes resin, reducing preparation time for screening and small-scale purification work
- Phenyl ligand chemistry, supporting hydrophobic interaction selectivity during binding and elution optimisation
- 40 µm particle size, with a 25 mm bed height in the 1 mL HiTrap format
- Process-relevant performance, with flow rate specified up to 220 cm/h under stated process buffer conditions
- 5 bar maximum pressure, equivalent to 0.5 MPa or 70 psi
- Broad working pH range, suitable for use from pH 3 to 13 with storage in 20% ethanol at 4 °C to 30 °C
Note: The temperature difference between the fluid running through the column and the ambient room temperature should never be greater than 20 °C.
Benefits
- Helps laboratories screen selectivity, binding and elution conditions before scaling purification methods.
- Supports intermediate and polishing protein purification where HIC selectivity is required.
- Prepacked columns reduce the need for manual column packing during early-stage method development.
- High binding capacity, high flow rate and low back pressure can help reduce process cycle times in suitable workflows.
- Chemical stability supports cleaning with standard CIP solutions, including 1 M sodium hydroxide.
Why Choose the HiTrap Capto Phenyl ImpRes Column, 5 x 1 mL (17548411)?
Choose the HiTrap Capto Phenyl ImpRes Column, 5 x 1 mL (17548411), when you need a convenient prepacked HIC column set for resin screening, condition optimisation and small-scale protein purification. John Morris Group can supply this HiTrap chromatography product for Australian laboratories working with chromatography method development and purification workflows.
