The HiTrap Capto Octyl HIC Column, 5 x 1 mL (17546508), is a pack of five prepacked 1 mL columns for hydrophobic interaction chromatography of proteins. Each column is prepacked with Capto Octyl resin, an HIC resin with an octyl ligand for capture and intermediate purification of monoclonal antibodies, mAb variants and other biomolecules.
Key Features
- Prepacked HiTrap format, supplied as 5 x 1 mL columns for convenient small-scale HIC purification
- Capto Octyl resin chemistry, using an octyl hydrophobic ligand for protein separation by surface hydrophobicity
- Hydrophobic interaction chromatography mode, suited to capture and intermediate purification of proteins and biomolecules
- Highly cross-linked agarose matrix, providing a spherical high-flow resin base with low backpressure
- Approximate 75 µm particle size, based on median particle size of the cumulative volume distribution
- BSA binding capacity around 25 mg/mL resin, measured at 10% breakthrough under Cytiva test conditions
- 1 mL HiTrap operating flow, with 1 mL/min flow rate listed for the exact 5 x 1 mL product format
- Broad pH compatibility, with operational pH stability from 3 to 13 and CIP stability from pH 2 to 14
Benefits
- Supports rapid screening of HIC selectivity, binding and elution conditions before scale-up.
- Provides ready-to-use column convenience without the need for small-column packing.
- Helps evaluate octyl ligand selectivity for proteins, mAbs, mAb variants and other biomolecules.
- Supports small-scale purification and process development using syringe, pump or chromatography system workflows.
- Allows repeat experiments across five matched 1 mL columns in one pack.
Why Choose the HiTrap Capto Octyl HIC Column, 5 x 1 mL (17546508)?
Choose the HiTrap Capto Octyl HIC Column, 5 x 1 mL (17546508), when you need a prepacked HIC column for screening hydrophobic interaction conditions and small-scale protein purification. John Morris Group supplies Cytiva chromatography products to Australian laboratories with local support for selection and ordering.
