HiTrap Capto Butyl ImpRes Columns, 5 × 5 mL (17371912) are ready-to-use prepacked columns containing Capto Butyl ImpRes hydrophobic interaction chromatography resin. This pack contains five 5 mL columns for screening selectivity, binding, and elution conditions, as well as small-scale protein purification.
Key Features
- Five prepacked 5 mL columns, supplied in a ready-to-use HiTrap format for small-scale purification work
- Hydrophobic interaction chromatography format, designed for screening and protein purification using HIC conditions
- Capto Butyl ImpRes resin chemistry, based on a high-flow agarose matrix with a butyl ligand
- 40 µm average particle size, supporting high-resolution intermediate and polishing purification
- 37 mg BSA/mL resin binding capacity, measured at 10% breakthrough under stated Cytiva test conditions
- Broad pH stability, with operational pH 3 to 13 and short-term CIP stability from pH 2 to 14
- 5 bar maximum pressure, with 16 mm internal diameter and 25 mm column height
- 20% ethanol storage condition, suitable for storage at 4°C to 30°C
Benefits
- Helps screen selectivity, binding, and elution conditions before moving to larger-scale HIC purification.
- Supports method development for intermediate and polishing steps in downstream protein purification workflows.
- Provides a convenient prepacked format that reduces column preparation time.
- Allows process developers to evaluate Capto Butyl ImpRes resin using a practical 5 mL column size.
- Supports cleaning strategies that include standard CIP conditions such as 1 M sodium hydroxide where suitable for the method.
Why Choose the HiTrap Capto Butyl ImpRes Columns, 5 × 5 mL (17371912)?
HiTrap Capto Butyl ImpRes Columns, 5 × 5 mL (17371912) are suited to laboratories developing HIC methods for small-scale protein purification and process optimisation. John Morris Group supplies HiTrap prepacked chromatography columns to support Australian laboratories with screening, method development, and scale-up workflows.
