HiTrap Protein L Columns, 1 × 1 mL (29048665) are prepacked affinity chromatography columns for purification of antibody fragments containing the kappa light chain. Each 1 mL HiTrap column is packed with Capto L resin, using recombinant protein L ligand for capture of suitable antibodies and antibody fragments such as Fab fragments, scFv, and Dabs.
Key Features
- 1 × 1 mL HiTrap format, supplied as one ready-to-use prepacked affinity chromatography column
- Capto L affinity resin, designed for capture of antibodies and antibody fragments containing suitable kappa light chain regions
- Recombinant protein L ligand, produced in E. coli and coupled to a rigid, highly cross-linked agarose matrix
- High antibody fragment binding capacity, with approximately 25 mg Fab/mL medium at 4 minute residence time
- 85 µm average particle size, supporting high-flow affinity chromatography operation
- 0.7 × 2.5 cm column dimensions, providing a compact 1 mL format for small-scale purification and optimisation
- Defined HiTrap pressure limit, rated at 0.5 MPa (5 bar) for the column hardware
Benefits
- Helps purify suitable antibody fragments in a ready-to-use format without manual column packing.
- Supports method optimisation and small-scale purification before moving to larger Capto L formats.
- Provides protein L selectivity for antibody fragments where kappa light chain binding is appropriate.
- Can be used with liquid chromatography systems such as ÄKTA for fast and reproducible separations.
Why Choose the HiTrap Protein L Columns, 1 × 1 mL (29048665)?
Choose the HiTrap Protein L Columns, 1 × 1 mL (29048665) when you need a prepacked Capto L affinity column for purification of antibody fragments containing the kappa light chain. John Morris Group can help confirm availability and support selection of related Cytiva chromatography consumables for Australian laboratories.
