The HiTrap MabSelect PrismA X Protein A Column, 5 x 5 mL (17550154), is a pack of prepacked HiTrap columns containing MabSelect PrismA X Protein A affinity resin. It is designed for efficient monoclonal antibody capture, with high dynamic binding capacity and exceptional alkaline stability for repeated cleaning-in-place workflows.
Key Features
- Ready-to-use HiTrap column format, prepacked with MabSelect PrismA X resin for Protein A affinity chromatography
- 5 x 5 mL pack size, supplied as five 5 mL columns for method development and small-scale purification work
- High-capacity mAb capture resin, designed to support efficient capture of monoclonal antibodies
- Highly cross-linked agarose matrix, supplied as spherical resin beads for packed-bed chromatography
- 50 µm median particle size, supporting use in short bed height and rapid cycling chromatography applications
- Alkali-stable Protein A-derived ligand, produced in E. coli and attached by single-point coupling
- High dynamic binding capacity, with more than 80 mg mAb/mL resin at 6 min residence time and more than 74 mg mAb/mL resin at 4 min residence time
- Strong cleaning-in-place compatibility, supporting use of 0.5 to 1.0 M sodium hydroxide for robust cleaning workflows
Benefits
- Reduces preparation time by supplying MabSelect PrismA X resin in a convenient prepacked column format.
- Helps process development teams evaluate high-capacity Protein A capture before moving to larger formats.
- Supports robust cleaning and sanitisation to help reduce bioburden risk between purification cycles.
- Can help increase mAb throughput per resin volume while reducing resin and buffer consumption.
- Provides a practical small-scale format for monoclonal antibody and selected bispecific antibody capture workflows.
Why Choose the HiTrap MabSelect PrismA X Protein A Column, 5 x 5 mL (17550154)?
The HiTrap MabSelect PrismA X Protein A Column, 5 x 5 mL (17550154), is suited to laboratories developing high-capacity antibody capture workflows using an alkali-stable Protein A resin. John Morris Group supplies Cytiva chromatography columns across Australia, helping customers select suitable prepacked columns for downstream purification and process development.
