The HiTrap MabSelect PrismA X Column, 5 x 1 mL (17550152), is a set of prepacked protein A affinity chromatography columns containing MabSelect PrismA X resin. The resin is designed for high-capacity monoclonal antibody capture and is well suited to short bed height applications using rapid cycling chromatography.
Key Features
- Prepacked 5 x 1 mL HiTrap format, supplied as five 1 mL columns for small-scale mAb capture and process development work
- High-capacity protein A resin, designed to efficiently capture monoclonal antibodies in affinity chromatography workflows
- Rapid cycling chromatography suitability, supporting short bed height applications for improved capture-step productivity
- Highly cross-linked spherical agarose matrix, providing the base structure for MabSelect PrismA X resin performance
- Alkali-stable protein A-derived ligand, produced in E. coli and attached using single-point coupling
- Approximately 50 µm median particle size, based on the cumulative volume distribution of the resin
- High mAb dynamic binding capacity, with more than 80 mg mAb/mL resin at 6 min residence time and more than 74 mg mAb/mL resin at 4 min residence time
- Broad cleaning and operating range, with pH 3 to 12 operational stability, pH 2 to 14 CIP stability, and 0.5 to 1.0 M NaOH CIP stability
Benefits
- Supports high-capacity mAb capture in a convenient prepacked column format.
- Helps reduce resin and buffer consumption through strong dynamic binding capacity.
- Allows rapid cycling chromatography studies using short bed height process strategies.
- Supports robust cleaning between runs using sodium hydroxide-based CIP conditions.
- Provides five 1 mL columns for screening, evaluation, and method development workflows.
Why Choose the HiTrap MabSelect PrismA X Column, 5 x 1 mL (17550152)?
Choose the HiTrap MabSelect PrismA X Column, 5 x 1 mL (17550152), when your workflow needs a compact prepacked protein A column format for high-capacity mAb capture and rapid cycling chromatography development. John Morris Group can support Australian laboratories with Cytiva chromatography product selection, ordering, and related workflow requirements.
