The HiScreen MabSelect PrismA Column, 4.7 mL (17549815) is a prepacked protein A affinity chromatography column for antibody purification, process development, and optimisation. It is packed with MabSelect PrismA resin, a next-generation protein A resin with an optimised high-flow agarose base matrix and an alkali-tolerant protein A-derived ligand.
Key Features
- Prepacked 4.7 mL HiScreen format, with a 10 cm bed height and narrow inner diameter for method optimisation and parameter screening
- MabSelect PrismA protein A resin, designed for improved antibody purification performance with enhanced alkaline stability and binding capacity
- Optimised agarose matrix, using rigid, highly cross-linked agarose with approximately 60 µm median particle size
- High dynamic binding capacity, with approximately 80 mg polyclonal IgG/mL resin at 6 min residence time
- Efficient alkaline cleaning, supporting cleaning between runs using 0.5 to 1.0 M NaOH
- Compact column geometry, with 0.77 x 10 cm dimensions and an 8 bar hardware pressure limit
- Defined operating flow rates, with recommended operating flow rate of 1.8 mL/min and maximum operating flow rate below 4.7 mL/min
- Wide pH stability range, with operational pH stability from 3 to 12 and CIP pH stability from 2 to 14
Benefits
- Supports fast and reproducible purification of antibodies in a ready-to-use prepacked format.
- Helps optimise process development conditions while keeping bed height and flow velocity relevant for scale-up.
- Allows more robust cleaning between purification runs, helping reduce carryover and cross-contamination risk.
- Provides high binding capacity to support improved antibody recovery during protein A capture.
- Can be used for preparative purification, screening, and optimisation of antibody purification conditions.
Why Choose the HiScreen MabSelect PrismA Column, 4.7 mL (17549815)?
The HiScreen MabSelect PrismA Column, 4.7 mL (17549815) is suited to laboratories developing monoclonal antibody and bispecific antibody purification workflows where alkaline stability, capacity, and scale-up relevance matter. John Morris Group supplies Cytiva prepacked chromatography columns to Australian laboratories and can assist with product selection, ordering, and availability.
