The HiTrap MabSelect VH3 Column, 5 × 1 mL (17549352), is a prepacked affinity chromatography column format containing MabSelect VH3 protein A resin. The resin uses an alkali-stable protein A-derived ligand engineered for interaction with the VH3 region rather than the Fc region. This 5 × 1 mL pack is suited to screening, method development, and small-scale purification of bispecific antibodies and antibody fragments such as Fab, scFv, and VHH when the VH3 sequence family is present.
Key Features
- Prepacked 1 mL HiTrap format, supplied as five columns for convenient small-scale affinity chromatography work
- VH3-specific affinity interaction, using a protein A-derived ligand with enhanced interaction with the VH3 region and no Fc-region interaction
- Highly cross-linked agarose matrix, supplied as spherical beads for chromatography resin performance
- Single-point ligand attachment, using epoxy coupling chemistry
- Defined particle size, with a median particle size of approximately 60 µm
- High dynamic binding capacity, with approximately 70 mg mAb/mL resin at 6 min residence time and approximately 60 mg mAb/mL resin at 4 min residence time under Cytiva test conditions
- Broad operating pH range, with operational stability from pH 3 to 12
- CIP-compatible pH stability, with cleaning-in-place stability from pH 2.5 to 13.7
Benefits
- Supports efficient screening of VH3 affinity purification conditions before moving to larger formats.
- Helps separate target bispecific antibodies from unwanted mispaired antibodies and fragments when VH3 binding behaviour is useful.
- Provides a ready-to-use column format that reduces manual packing time and improves workflow convenience.
- Supports purification development for antibody formats that may not be efficiently captured by conventional Fc-focused protein A workflows.
- Allows laboratories to evaluate MabSelect VH3 selectivity using low-volume, prepacked columns.
Why Choose the HiTrap MabSelect VH3 Column, 5 × 1 mL (17549352)?
The HiTrap MabSelect VH3 Column, 5 × 1 mL (17549352), is a practical format for evaluating MabSelect VH3 resin in small-scale affinity chromatography workflows. John Morris Group supplies Cytiva chromatography columns to Australian laboratories with support for product selection and ordering.
