The HiTrap MabSelect VH3 Column, 1 x 1 mL (17549351), is a prepacked affinity chromatography column containing MabSelect VH3 protein A resin. The engineered ligand interacts only with the variable heavy chain of the VH3 sequence family of human antibodies, supporting efficient separation of bispecific antibodies, Fab, scFv, and VHH formats that contain the VH3 sequence family.
Key Features
- Prepacked 1 x 1 mL HiTrap format, supplied as a 1 mL column for small-scale affinity purification and process development
- VH3-selective protein A interaction, with no Fc region interaction and enhanced interaction with the VH3 region
- Designed for antibody variants and fragments, including bispecific antibodies, Fab, scFv, and VHH formats containing the VH3 sequence family
- Highly cross-linked spherical agarose matrix, supporting robust affinity chromatography performance
- Alkali-stable protein A-derived ligand, coupled by single-point attachment using epoxy chemistry
- Approximately 60 µm particle size, based on the median particle size of the cumulative volume distribution
- High mAb binding capacity, with approximately 70 mg mAb/mL resin at 6 min residence time and approximately 60 mg mAb/mL resin at 4 min residence time
- Broad pH stability range, with pH 3 to 12 operational stability and pH 2.5 to 13.7 cleaning-in-place stability
Benefits
- Supports efficient capture and separation of VH3-containing antibody fragments and bispecific antibodies.
- Helps resolve product-related impurities during affinity capture workflows.
- Reduces preparation time by providing MabSelect VH3 resin in a prepacked HiTrap column format.
- Supports cleaning with 0.5 M NaOH, helping laboratories maintain robust purification workflows.
Why Choose the HiTrap MabSelect VH3 Column, 1 x 1 mL (17549351)?
Choose the HiTrap MabSelect VH3 Column, 1 x 1 mL (17549351), when your workflow needs a compact prepacked affinity column for VH3-selective antibody purification and method development. John Morris Group can support Australian laboratories with Cytiva chromatography product selection, ordering, and related workflow requirements.
