The HiScreen MabSelect VH3 Column, 1 x 4.7 mL (17549315), is a prepacked affinity chromatography column containing MabSelect VH3 protein A resin. The ligand is engineered to interact with the variable heavy chain of the VH3 sequence family while removing Fc interaction, supporting purification of VH3-containing bispecific antibodies and fragments such as Fab, scFv and VHH.
Key Features
- Prepacked 1 x 4.7 mL HiScreen format, supplied for small-scale purification and process development with MabSelect VH3 resin
- VH3-specific protein A-derived ligand, engineered for enhanced interaction with the VH3 region and no interaction with the Fc region
- Affinity antibody purification use, suitable for VH3-containing bispecific antibodies, Fab, scFv and VHH fragments
- Highly cross-linked agarose matrix, supplied as spherical resin beads with an approximate 60 µm particle size
- Single-point ligand attachment, using epoxy coupling chemistry for the MabSelect VH3 ligand
- High dynamic binding capacity, with approximately 70 mg mAb/mL resin at 6 min residence time
- Defined operating stability, with pH 3 to 12 for operation and pH 2.5 to 13.7 for cleaning-in-place
- Recommended high-flow operation, with maximum operating flow velocity of 300 cm/h at 20 cm bed height or 550 cm/h at 10 cm bed height
Benefits
- Supports efficient separation of VH3-containing bispecific antibodies and antibody fragments during affinity capture.
- Helps reduce unwanted Fc-based binding by using a ligand with the Fc interaction removed.
- Provides a convenient prepacked format for screening, method development and scale-up preparation.
- High binding capacity can support strong mass throughput per resin volume in suitable workflows.
- Alkaline stability supports cleaning-in-place conditions for robust purification development.
Why Choose the HiScreen MabSelect VH3 Column, 1 x 4.7 mL (17549315)?
Choose the HiScreen MabSelect VH3 Column, 1 x 4.7 mL (17549315), when you need a prepacked affinity column for VH3-specific antibody and fragment purification. John Morris Group can supply this HiScreen chromatography product for Australian laboratories developing purification workflows for bispecific antibodies, Fab, scFv and VHH targets.
