The MabSelect VH3 Protein A Affinity Resin, 200 mL (17549302) is a protein A affinity chromatography resin for capture and separation of VH3-containing antibodies, bispecific antibodies, and antibody fragments. It uses an alkali-stable protein A-derived ligand engineered for VH3-region interaction without Fc-region binding, supplied here as a 200 mL resin pack.
Key Features
- VH3-selective engineered ligand, with protein A-derived interaction enhanced for the VH3 region and no interaction with the Fc region
- High dynamic binding capacity, with QB10% of approximately 70 mg mAb/mL resin at 6 min residence time and approximately 60 mg mAb/mL resin at 4 min
- Rigid agarose bead format, using a highly cross-linked spherical agarose matrix with approximately 60 µm median particle size
- High-flow operation, supporting recommended maximum operating flow velocity of 300 cm/h at 20 cm bed height or 550 cm/h at 10 cm bed height
- Wide operating pH window, with operational pH stability from 3 to 12 and CIP pH stability from 2.5 to 13.7
- Delivered in storage solution, supplied in 20% ethanol in a 200 mL pack
Benefits
- Helps target VH3 sequence family interactions for efficient capture of bispecific antibodies and antibody fragments.
- Supports separation strategies where Fc-region interaction is not desired.
- Offers high binding capacity to support productive affinity chromatography workflows.
- Provides a broad pH operating and CIP range for robust cleaning and process handling.
- Combines high-flow operation with a rigid agarose matrix for scalable chromatography performance.
Why Choose the MabSelect VH3 Protein A Affinity Resin, 200 mL (17549302)?
The MabSelect VH3 Protein A Affinity Resin, 200 mL (17549302) is designed for VH3-focused antibody purification where selectivity, binding capacity, and alkaline stability are important. John Morris Group supplies Cytiva chromatography resins to Australian laboratories and can assist with product selection, ordering, and availability.
