The HiTrap Protein L Antibody Fragment Column, 5 x 1 mL (17547851) is a pack of prepacked affinity chromatography columns for purification of antibody fragments containing kappa light chains. Packed with Capto L resin, it combines a rigid, high-flow agarose matrix with a recombinant Protein L ligand for convenient small-scale purification and method development.
Key Features
- Prepacked HiTrap format, supplied as 5 x 1 mL columns for small-scale antibody fragment purification
- Capto L affinity resin, designed for capture of antibodies and antibody fragments containing kappa light chains
- Kappa light chain binding specificity, using recombinant Protein L with affinity for the variable region of antibody kappa light chains
- Suitable for antibody fragments, including Fab fragments, scFv, and domain antibodies
- Rigid high-flow agarose matrix, supporting fast and reproducible separations in a convenient column format
- 85 µm median particle size, based on the d50v average particle size of the Capto L resin
- Dynamic binding capacity around 25 mg Fab/mL medium, determined at 10% breakthrough under stated test conditions
- Convenient system connection, suitable for use with a syringe, peristaltic pump, or chromatography system such as an ÄKTA system
Benefits
- Supports efficient purification of antibody fragments where kappa light chain binding is suitable.
- Helps simplify early-stage purification by using a ready-to-use prepacked column format.
- Reduces development time by supporting method optimisation and small-scale purification without manual packing.
- Provides consistent column performance for screening binding, wash, and elution conditions.
- Supports scale-up planning using the same Capto L resin chemistry available in other formats.
Why Choose the HiTrap Protein L Antibody Fragment Column, 5 x 1 mL (17547851)?
The HiTrap Protein L Antibody Fragment Column, 5 x 1 mL (17547851) is suited to laboratories purifying antibody fragments that contain kappa light chains. John Morris Group supplies Cytiva HiTrap columns and chromatography resins for Australian laboratories and can help confirm suitability for your antibody fragment purification workflow.
