The HiTrap Protein L Antibody Fragment Column, 1 x 5 mL (17547815), is a prepacked affinity chromatography column for purification of antibody fragments containing the kappa light chain. It is packed with Capto L resin, which uses recombinant protein L ligand on a rigid, highly cross-linked agarose matrix for capture of Fabs, scFv fragments and domain antibodies.
Key Features
- Prepacked HiTrap format, supplied as 1 x 5 mL for small-scale purification and method development
- Protein L affinity chemistry, using recombinant protein L ligand with affinity for the variable region of antibody kappa light chains
- Capto L resin base, combining a rigid, highly cross-linked agarose matrix with high-flow performance
- 85 µm average particle size, based on d50v median particle size of the cumulative volume distribution
- Approximate Fab binding capacity of 25 mg/mL medium, measured at 10% breakthrough and 4 min residence time
- 5 mL HiTrap operating flow, with recommended flow rate of 5 mL/min and maximum flow rate of 20 mL/min
- 5 bar column pressure limit, equivalent to 0.5 MPa or 70 psi for the HiTrap column hardware
- Working pH range of 2 to 10, with recommended cleaning-in-place using 15 mM NaOH
Benefits
- Supports affinity capture of antibody fragments that contain suitable kappa light chain binding regions.
- Provides a ready-to-use column format for small-scale purification without manual column packing.
- Helps screen sample load, binding conditions and elution conditions before scale-up or further optimisation.
- Can support platform-style purification of Fabs, scFv fragments and domain antibodies where Protein L binding is suitable.
- Offers convenient use with syringe, pump or compatible chromatography system workflows.
Why Choose the HiTrap Protein L Antibody Fragment Column, 1 x 5 mL (17547815)?
Choose the HiTrap Protein L Antibody Fragment Column, 1 x 5 mL (17547815), when you need a prepacked Capto L column for small-scale antibody fragment purification by Protein L affinity chromatography. John Morris Group supplies Cytiva chromatography products to Australian laboratories with local support for selection and ordering.
