The HiScreen MabSelect SuRe LX Column, 1 × 4.7 mL (17547415) is a prepacked protein A affinity chromatography column for monoclonal antibody capture in process development. Packed with MabSelect SuRe LX resin, the 4.7 mL HiScreen format supports method optimisation, parameter screening and robustness testing where high dynamic binding capacity is required.
Key Features
- Prepacked 1 × 4.7 mL HiScreen format, supplied as one ready-to-use column for antibody capture process development
- High dynamic binding capacity, with approximately 60 mg human IgG/mL medium at 6 min residence time
- Alkali-stabilised protein A-derived ligand, derived from E. coli and designed for antibody affinity capture
- Rigid highly cross-linked agarose matrix, supporting high-flow operation in protein A chromatography workflows
- 85 µm median particle size, providing a defined resin format for scalable process development
- Defined HiScreen geometry, with 4.7 mL column volume, 0.77 × 10 cm column dimensions and 10 cm bed height
- Useful cleaning-in-place stability, allowing 0.1 to 0.5 M NaOH for CIP under stated conditions
- Scalable development format, two HiScreen columns can be connected in series to achieve a 20 cm bed height
Benefits
- Helps screen binding, wash and elution conditions while keeping sample and buffer consumption low.
- Supports development of high-capacity protein A capture steps for high-titre antibody feeds.
- Provides a convenient prepacked column format, reducing manual packing work during early process development.
- Enables scale-up studies using the same linear fluid velocity as larger BioProcess columns packed with the same resin.
Why Choose the HiScreen MabSelect SuRe LX Column, 1 × 4.7 mL (17547415)?
Choose the HiScreen MabSelect SuRe LX Column, 1 × 4.7 mL (17547415) when you need a compact prepacked protein A column for high-DBC monoclonal antibody capture and process development. John Morris Group supplies Cytiva chromatography consumables for Australian laboratories and can help confirm suitability for your antibody purification workflow.
