The MabSelect SuRe 70 HiTrap Column, 1 × 5 mL (17542413) is a prepacked protein A affinity chromatography column for monoclonal antibody capture. Packed with MabSelect SuRe 70 resin, the column combines high dynamic binding capacity, an alkali-stable protein A-derived ligand and a 50 µm high-flow agarose matrix for productive mAb purification workflows.
Key Features
- Prepacked 1 × 5 mL HiTrap format, supplied as one 5 mL column for antibody affinity chromatography
- High dynamic binding capacity, with greater than 70 mg mAb/mL resin at 6 min residence time and greater than 65 mg mAb/mL resin at 4 min residence time
- Defined 5 mL column geometry, with 16 × 25 mm bed dimensions, 16 mm column i.d. and 25 mm bed height
- Highly cross-linked agarose matrix, spherical and designed for protein A affinity chromatography applications
- 50 µm median particle size, supporting high-flow operation and fast mAb uptake
- Alkali-stable protein A-derived ligand, derived from E. coli and attached by single-point coupling
- Short bed height flow capability, with recommended maximum operating flow velocity of 220 cm/h at 20 cm bed height and 400 cm/h at 10 cm bed height
- Broad cleaning and operating window, with operational pH stability of 3 to 12 and CIP pH stability of 2 to 13.7
Benefits
- Helps improve capture-step productivity by maintaining high mAb binding capacity at practical residence times.
- Supports cost-efficient antibody capture where strong binding capacity and stable alkaline cleaning are important.
- Provides a ready-to-use 5 mL HiTrap column format, reducing the need for manual resin packing at lab scale.
- Enables rapid resin cycling and short bed height strategies for higher throughput protein A capture workflows.
Why Choose the MabSelect SuRe 70 HiTrap Column, 1 × 5 mL (17542413)?
Choose the MabSelect SuRe 70 HiTrap Column, 1 × 5 mL (17542413) when you need a prepacked protein A column for high-DBC monoclonal antibody capture using MabSelect SuRe 70 resin. John Morris Group supplies Cytiva chromatography consumables for Australian laboratories and can help confirm suitability for your antibody purification workflow.
