PreDictor RoboColumn Ni Sepharose Excel, 200 µL, Row of 8 (29714915) is a prepacked minicolumn format for high-throughput chromatography process development. This variant is packed with Ni Sepharose Excel affinity resin and is used for IMAC purification development of histidine-tagged proteins.
Key Features
- 200 µL column volume, with a 10.0 mm bed height for small-scale process development studies
- Prepacked Ni Sepharose Excel format, supporting IMAC screening for his-tagged protein purification workflows
- Suitable for samples containing nickel stripping agents, supporting purification development with challenging eukaryotic cell culture supernatants
- One row of eight columns, supplied in a format suited to parallel experimental design
- Polypropylene column and filter materials, supporting routine use with compatible chromatography buffers
- 5 mm internal diameter, with a 39 mm column height in the 200 µL column format
- 7 to 12 µm filter pore size, built into the PreDictor RoboColumn unit design
- Broad working range, with operation from 4°C to 30°C and flow velocity from 16 to 1000 cm/h
Benefits
- Allows multiple IMAC purification conditions to be screened in a compact, prepacked minicolumn format.
- Helps assess binding, wash and elution conditions before moving to larger verification columns.
- Reduces manual preparation work by using prepacked columns in a row format.
- Supports early-stage his-tagged protein purification development where small sample volumes and parallel testing are important.
Why Choose the PreDictor RoboColumn Ni Sepharose Excel, 200 µL, Row of 8 (29714915)?
This PreDictor RoboColumn Ni Sepharose Excel variant gives process development teams a compact, prepacked format for IMAC chromatography screening. John Morris Group can help Australian laboratories source Cytiva chromatography consumables and confirm suitability for the intended workflow.
