The PreDictor RoboColumn Protein G Sepharose 4 FF, 200 µL (29275288) is a miniaturised prepacked chromatography column for high-throughput process development. It is packed with Protein G Sepharose 4 Fast Flow, an affinity chromatography resin for capture of mammalian immunoglobulin G and subclasses.
Key Features
- Prepacked Protein G Sepharose 4 FF minicolumn, using an affinity chromatography resin for IgG capture workflows
- 200 µL column volume, with a 10.0 mm bed height for small sample consumption
- Miniaturised RoboColumn format, designed for automated and parallel chromatographic separations
- Protein G affinity ligand, recombinant streptococcal protein G lacking the albumin-binding region and produced in E. coli
- Cross-linked agarose matrix, using 4% spherical agarose with an approximately 90 µm particle size
- Human IgG binding capacity, specified at greater than or equal to 20 mg human IgG/mL resin
- Defined pH stability, with operational pH stability from 3 to 9 and CIP pH stability from 2 to 10
- Array plate workflow, supplied in rows of eight columns that can be arranged on a 96-position array plate
Note: Avoid halogenated organic solvents and hexane with PreDictor RoboColumn units.
Benefits
- Helps reduce sample consumption during antibody affinity resin and condition screening.
- Supports faster experimental setup by enabling automated, parallel chromatography workflows.
- Allows larger experimental spaces to be explored for improved process understanding.
- Supports capture or purification method development for mammalian IgG and subclasses.
- Provides a compact format for screening Protein G Sepharose 4 Fast Flow affinity chromatography conditions.
Why Choose the PreDictor RoboColumn Protein G Sepharose 4 FF, 200 µL (29275288)?
Choose the PreDictor RoboColumn Protein G Sepharose 4 FF, 200 µL (29275288) when you need a miniaturised prepacked Protein G affinity column for high-throughput antibody capture method development. John Morris Group can supply this Cytiva consumable for Australian laboratories and help confirm suitability for your robotic liquid handling and chromatography workflow.
