PreDictor RoboColumn Butyl Sepharose 4FF, 200 µL (28986100) is a miniaturised prepacked column format containing Butyl Sepharose 4 Fast Flow resin for hydrophobic interaction chromatography process development. These 200 µL minicolumns are supplied as one row of eight columns and support high-throughput screening for capture and intermediate purification of large proteins.
Key Features
- Prepacked RoboColumn minicolumn format, designed for automated parallel chromatography experiments in high-throughput process development
- 200 µL bed volume, with a 10.0 mm bed height for miniaturised chromatography screening
- Hydrophobic interaction chromatography resin, using Butyl Sepharose 4 Fast Flow for capture and intermediate purification workflows
- Butyl ligand chemistry, providing hydrophobic selectivity for protein separation studies
- Approx. 50 µmol butyl/mL gel degree of substitution, supporting consistent Butyl Sepharose 4 Fast Flow resin behaviour
- Highly cross-linked 4% agarose matrix, with spherical beads for Fast Flow resin performance
- 90 µm mean particle size, with a 45 to 165 µm bead size range for Sepharose Fast Flow media
- Pack of eight minicolumns, supplied as one row of eight 200 µL RoboColumn units
Benefits
- Helps laboratories screen hydrophobic interaction chromatography conditions in parallel before scaling to larger column formats.
- Reduces sample and buffer consumption during early process development studies.
- Supports evaluation of salt, pH, binding, wash, and elution conditions for protein purification workflows.
- Provides a practical format for assessing Butyl Sepharose 4 Fast Flow performance in capture and intermediate purification development.
Why Choose the PreDictor RoboColumn Butyl Sepharose 4FF, 200 µL (28986100)?
PreDictor RoboColumn Butyl Sepharose 4FF, 200 µL (28986100) provides a compact, high-throughput format for screening hydrophobic interaction chromatography conditions with Butyl Sepharose 4 Fast Flow resin. John Morris Group supplies this Cytiva consumable for Australian laboratories developing efficient biomolecule purification workflows.
