The PreDictor Capto L Plate, 50 µL (17547832) is a pack of four 96-well filter plates prefilled with Capto L affinity chromatography resin. Each well contains 50 µL of sedimented resin, providing a high-throughput format for screening capture conditions for antibodies and antibody fragments that contain kappa light chains.
Key Features
- Prefilled 96-well filter plate format, supplied as four plates for high-throughput process development studies
- 50 µL sedimented resin per well, using Capto L resin in a consistent single-resin plate format
- Protein L affinity chemistry, using a recombinant Protein L ligand produced in E. coli
- Kappa light-chain selectivity, binding to the variable region of antibody kappa light chains
- Rigid highly cross-linked agarose matrix, supporting high-flow affinity chromatography workflows
- Approx. 25 mg Fab/mL resin dynamic binding capacity, measured at 10% breakthrough under stated test conditions
- 800 µL well volume, with a recommended working volume of 100 to 300 µL when using a microplate shaker
- Plate construction in polypropylene and polyethylene, with an ANSI/SBS footprint of 127.8 x 30.6 x 85.5 mm
Benefits
- Enables rapid parallel screening of binding, wash and elution conditions using small sample volumes.
- Helps shorten antibody fragment process development by evaluating many chromatography conditions in a 96-well format.
- Supports capture studies for antibody fragments such as Fab fragments, scFv and Dabs where kappa light-chain binding is suitable.
- Provides a useful low-volume screening step before moving to Capto L column or bulk resin formats.
- Supports flexible manual or automated workflows using centrifugation, vacuum filtration or microplate shaker incubation.
Why Choose the PreDictor Capto L Plate, 50 µL (17547832)?
The PreDictor Capto L Plate, 50 µL (17547832) gives Australian laboratories a high-throughput format for evaluating Protein L affinity capture of antibodies and antibody fragments. John Morris Group supplies Cytiva chromatography products with local support to help match plate format, resin chemistry and process development workflow to your application.
