The PreDictor Capto L Plate, 6 µL (17547830) is a 96-well filter plate prefilled with Capto L protein L affinity chromatography resin. Supplied as 4 x 96-well filter plates, it is designed for high-throughput process development workflows for antibodies and antibody fragments containing kappa light chains.
Key Features
- Prefilled 6 µL resin format, with Capto L resin supplied in each well for single-resin affinity chromatography studies
- 4 x 96-well filter plate pack, supporting parallel screening of binding, wash, and elution conditions
- Protein L affinity chemistry, binding to the variable region of antibody kappa light chains
- Antibody fragment capture, suitable for fragments such as Fabs, single-chain variable fragments, and domain antibodies
- Disposable polypropylene and polyethylene construction, with 96 wells and an 800 µL total well volume
- Miniaturised working volume, with 100 to 300 µL recommended working volume per well when incubating on a microplate shaker
- Rigid high-flow agarose matrix, using recombinant protein L from E. coli coupled by epoxy chemistry
- Useful resin binding capacity, with dynamic binding capacity of approximately 25 mg Fab per mL medium under specified breakthrough conditions
Benefits
- Helps reduce process development time by allowing multiple affinity purification conditions to be screened in parallel.
- Supports lower sample consumption compared with early screening in larger packed-column formats.
- Provides a practical small-scale format for studying antibody fragment capture before column optimisation.
- Supports scale-up planning because Capto L resin is also available in prepacked column and bulk resin formats.
Why Choose the PreDictor Capto L Plate, 6 µL (17547830)?
The PreDictor Capto L Plate, 6 µL (17547830) is a useful option for laboratories screening protein L affinity conditions for antibody fragments containing kappa light chains. John Morris Group supplies Cytiva chromatography consumables to Australian laboratories, helping users source verified plates for downstream purification method development.
