The PreDictor RoboColumn MabSelect VL Minicolumn, 600 µL (17542034) is a prepacked protein L affinity minicolumn for high-throughput process development. It contains MabSelect VL resin for affinity capture of antibody Fabs, bispecifics and other antibody variants containing a kappa light chain.
Key Features
- 600 µL RoboColumn format, supplied as one row of eight prepacked minicolumns for automated parallel chromatography
- MabSelect VL protein L resin, designed for affinity capture of antibody variants containing a kappa light chain
- Compact 5 mm internal diameter column geometry, with 30.0 mm bed height and 59 mm column height for the 600 µL format
- Rigid agarose protein L resin, using an alkaline-stabilised protein L-derived ligand on a highly cross-linked agarose matrix
- High dynamic binding capacity, with approximately 70 mg IgGκ1/mL resin at 6 minutes residence time and approximately 60 mg/mL at 4 minutes residence time
- Automation-ready configuration, arranged for 96-position array plate workflows using compatible robotic liquid handling systems
- Defined operating window, with up to 8.0 bar maximum working pressure and 16 to 1000 cm/h flow velocity for PreDictor RoboColumn units
- Polypropylene column and filter construction, with 7 to 12 µm filter pore size and 20% ethanol storage solution
Benefits
- Supports high-throughput screening of protein L affinity capture conditions using small sample volumes.
- Helps evaluate binding, washing and elution parameters before further optimisation in larger column formats.
- Provides a convenient scale-down format for process development where automated parallel testing is required.
- Can reduce manual workload by enabling parallel chromatographic separations on compatible robotic platforms.
- Supports antibody variant workflows where protein A capture may not be suitable or sufficient.
Why Choose the PreDictor RoboColumn MabSelect VL Minicolumn, 600 µL (17542034)?
The PreDictor RoboColumn MabSelect VL Minicolumn, 600 µL (17542034) provides a verified Cytiva format for small-volume, automated protein L affinity capture screening. John Morris Group supplies Cytiva chromatography consumables to Australian laboratories with local sourcing support and product assistance.
