The PreDictor RoboColumn MabSelect VL Minicolumn, 200 µL (17542033) is a prefilled protein L affinity minicolumn for high-throughput process development. It is packed with MabSelect VL resin for capture of antibody Fabs, bispecific antibodies, and other antibody variants containing a kappa light chain.
Key Features
- Prefilled 200 µL RoboColumn format, supplied as one row of eight minicolumns for parallel chromatography screening
- MabSelect VL protein L resin, designed for capture of antibody fragments, bispecific antibodies, and kappa light chain variants
- Miniaturised chromatography column design, supporting dynamic experiments when sample is limited
- High-throughput workflow compatibility, intended for automated parallel experiments using a robotic liquid handling system such as Freedom EVO
- Rigid highly cross-linked agarose matrix, supporting high-flow affinity chromatography screening
- Alkaline stabilised protein L-derived ligand, recombinantly produced in E. coli and engineered for improved alkaline stability
- High dynamic binding capacity, with approximately 60 mg IgGκ1/mL resin at 4 minutes residence time and approximately 70 mg IgGκ1/mL resin at 6 minutes residence time
- Controlled 60 µm resin particle size, based on the median particle size of the cumulative volume distribution
Benefits
- Supports rapid screening of chromatographic conditions using small sample volumes.
- Helps explore larger experimental spaces for improved process understanding during HTPD work.
- Provides a small-scale dynamic column format before further optimisation in HiTrap or HiScreen columns.
- Can reduce manual handling when used in automated and parallel purification workflows.
- Offers a protein L capture option for antibody variants that do not bind effectively to protein A.
Why Choose the PreDictor RoboColumn MabSelect VL Minicolumn, 200 µL (17542033)?
Choose the PreDictor RoboColumn MabSelect VL Minicolumn, 200 µL (17542033) when you need a small-volume protein L affinity column for automated screening of kappa light chain antibody capture conditions. John Morris Group supplies Cytiva chromatography products for Australian laboratories and can assist with selection, ordering, and related chromatography requirements.
