The HiTrap Capto Core 700 Columns, 5 x 1 mL (17548151), are prepacked chromatography columns containing Capto Core 700 multimodal BioProcess resin. They are designed for intermediate purification and polishing of viruses and other large biomolecules in flowthrough mode, with a 1 mL column volume per column.
Key Features
- Prepacked 5 x 1 mL HiTrap format, supplied as five 1 mL columns for convenient screening and small-scale purification
- Core bead multimodal resin, with an inactive outer layer and ligand-activated core for impurity capture
- Mr 700 000 outer layer size cutoff, allowing larger target molecules to pass in the flowthrough while smaller impurities enter the bead core
- Octylamine ligand chemistry, combining hydrophobic and positively charged interaction modes
- 85 µm average particle size, based on the cumulative volume distribution of the Capto Core 700 resin
- Approximately 13 mg ovalbumin/mL medium binding capacity, determined at 10% breakthrough with a 3 min residence time
- 500 cm/h maximum flow velocity, measured using water at room temperature
- Broad operating window, with pH 2 to 14 working range and pH 3 to 13 cleaning-in-place stability
Benefits
- Supports efficient polishing where the target virus or large biomolecule is collected in the flowthrough fraction.
- Helps remove smaller impurities using a multimodal core that binds across a wide pH and salt concentration range.
- Provides a ready-to-use small column format for method screening before further optimisation or scale-up.
- Reduces preparation time compared with packing small chromatography columns manually.
Why Choose the HiTrap Capto Core 700 Columns, 5 x 1 mL (17548151)?
Choose the HiTrap Capto Core 700 Columns, 5 x 1 mL (17548151), when your workflow needs a prepacked multimodal column for intermediate purification and polishing of viruses or other large biomolecules. John Morris Group can support Australian laboratories with Cytiva chromatography product selection, ordering, and related workflow requirements.
