HiScreen Capto Core 400 Column, 4.7 mL (17372410) is a ready-to-use prepacked HiScreen column containing Capto Core 400 multimodal chromatography resin. It combines size exclusion and binding chromatography in one resin, supporting intermediate purification and polishing of viruses and other large biomolecules in flowthrough mode.
Key Features
- Ready-to-use 4.7 mL HiScreen format, supplied as one prepacked column for process development and method optimisation
- Dual-function Capto Core 400 resin, combining size exclusion behaviour with multimodal binding inside the bead core
- Core bead technology, with an inactive shell that excludes larger target molecules while smaller impurities enter and bind
- Octylamine multimodal ligand, providing hydrophobic and positively charged binding interactions inside the bead core
- Mr 400 000 average molecular weight cutoff, allowing larger entities to pass through while smaller contaminants are captured
- 90 µm average particle size, supporting high-flow purification with reduced back pressure compared with smaller bead formats
- 22 mg ovalbumin/mL binding capacity, measured at 10% breakthrough with 3 min residence time on HiScreen columns
- Defined HiScreen column hardware, with 0.77 × 10 cm dimensions and an 8 bar column hardware pressure limit
Note: HiScreen columns cannot be opened or repacked. Avoid using oxidizing agents and citrate buffers with Capto Core 400 resin.
Benefits
- Supports efficient impurity removal while target viruses or other large biomolecules are collected in the flowthrough.
- Helps reduce the productivity limits often associated with traditional size exclusion chromatography.
- Allows method development using a small 4.7 mL column volume while keeping a process-relevant 10 cm bed height.
- Can be connected in series with another HiScreen column when a 20 cm bed height is required for development work.
- Provides a practical format for screening and optimising virus polishing workflows before scale-up.
Why Choose the HiScreen Capto Core 400 Column, 4.7 mL (17372410)?
HiScreen Capto Core 400 Column, 4.7 mL (17372410) gives process development teams a compact prepacked format for evaluating Capto Core 400 in virus and large biomolecule polishing workflows. John Morris Group supplies this Cytiva HiScreen column for Australian laboratories requiring verified chromatography consumables for downstream purification development.
